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10.1248/bpb.23.149 3 AraseY.IkedaK.MurashimaN.ChayamaK.TsubotaA.KoidaI.et al (1997)

When a protocol requires investigation of multiple mechanisms simultaneously, sourcing each compound individually introduces additional variables: separate reconstitution conditions, independent storage requirements, and manual management of component ratios during preparation

Then, 500 L of the supernatant was removed, 500 L of isopropyl alcohol was added (supernatant:isopropyl alcohol = 1:1), the mixture was allowed to stand for 5 min, and the mixture was centrifuged at 12,000 rpm at 4 for 10 min